Thermodynamic Analysis of Enzyme–Substrate Interactions by Isothermal Titration Calorimetry (ITC)
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Quick Start: Isothermal Titration Calorimetry (ITC)
Isothermal titration calorimetry (ITC) is a technique used to determine the thermodynamic properties of a chemical or physical equilibria. While the most abundant applications lie in the field of biochemical characterization, its use has increased in other fields because of minimal sample and buffer restrictions. The foundation of this technique is based on the simple measurement of heat, q. Be...
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Cooperative binding pervades Nature. This review discusses the use of isothermal titration calorimetry (ITC) in the identification and characterisation of cooperativity in biological interactions. ITC has broad scope in the analysis of cooperativity as it determines binding stiochiometries, affinities and thermodynamic parameters, including enthalpy and entropy in a single experiment. Examples ...
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ژورنال
عنوان ژورنال: Kagaku To Seibutsu
سال: 2015
ISSN: 0453-073X,1883-6852
DOI: 10.1271/kagakutoseibutsu.53.834